In most colonies, ants work in service of a single reproductive queen, but that's not always the way fire ant societies function.
Researchers have discovered colonies of tropical fire ants, insects native to Florida and coastal Georgia, living under the rule of multiple queens.
Scientists discovered the multi-queen colonies situated next to single-queen colonies.
"The coexistence of two dramatically different social structures fascinated me," researcher Kip Lacy, graduate fellow at Rockefeller University, said in a news release. "I had to know more."
Lacy made the discovery while earning his master's degree in entomology at the University of Georgia.
Kip Lacy. Credit: Andrew Davis Tucker/UGA
Lacy worked with colleagues at the U.S. Department of Agriculture Agricultural Research Service in Gainesville, Florida, to help find and isolate the communities of native fire ants that cover the shoulders and medians of Florida highways.
During their survey of fire ant colonies, Lacy and his colleagues, found single-queen colonies just a few feet away from colonies boasting a matriarchy of 13 queens.
"They can exist right next to each other, but their social structure remains intact," Lacy said.
Many fire ant colonies are founded by a single queen, mated to a male ant from a different colony, who produces all of the eggs to start a colony of her own. However, it's not unheard of for ants of the same species to develop alternative reproductive habits.
One of Lacy's colleagues, Ken Ross, a researcher at the University of Tennessee, previously identified multi-queen or "polygyne" colonies while studying Georgia's invasive red imported fire ants. He determined the multi-queen behavior was triggered by a "social chromosome," a genetic mutation that causes "polygyne syndrome."
Ross also found members of polygyne colonies were smaller in size and demonstrated lower levels of aggression toward unfamiliar ants. But in both types of red imported fire ant colonies, female offspring -- whether queens or workers -- are produced sexually.
Conversely, queens in polygyne colonies of tropical fire ants reproduce asexually. When Lacy and his colleagues conducted genetic analysis, they found all reproductive queens and daughter queens within polygyne colonies boasted the exact same genetic makeup.
Worker ants inside polygyne colonies featured significant genetic diversity, proving they are not clones.
"That was a surprise because we also found that the male ants in these colonies seem to be sterile," Lacy said.
Researchers determined queens from multi-queen colonies mate with males from single-queen colonies to maintain the colony's genetic diversity.
"The males get nothing out of it because their sperm goes to produce workers that are sterile," Ross said. "Those males' genetic lines end there."
Lacy, Ross and the rest of the team dubbed the behavior "sperm parasitism." They described their discovery this month in the journal Current Biology.
